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Status
Public on Apr 24, 2008
Title
wild type replicate 2 light
Sample type
RNA
Source name
7 day old seedlings
Organism
Arabidopsis thaliana Characteristics
wild type, 7 day old seedlings, light grown
Extracted molecule
total RNA
Extraction protocol
Approximately 80 mg from 7 day old light grown CSN4-1 in Col wild type seedlings were collected and ground to a fine powder under liquid Nitrogen. Samples were lysed in separate groups in RLT buffer (Qiagen).Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
Label
biotin
Label protocol
Approximately 6 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
CSN4-1 wild type 7 day old seedlings
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 15, 2005
Last update date
Aug 28, 2018
Contact name
Claus Schwechheimer
E-mail(s)
[email protected] Organization name
TUM
Department
Plant Systems Biology
Street address
Emil-Ramann-Str. 4
City
Freising
ZIP/Postal code
85354
Country
Germany
Platform ID
GPL198 Series (2)
GSE3865 CSN4-1 mutant analysis
GSE9728 COP9 signalosome (csn) mutant analysis
Relations Reanalyzed by
GSE118579 Reanalyzed by
GSE119083
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was P (P), A (A), M (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
485.1
P
0.00011
AFFX-BioB-M_at
630.4
P
0.00004
AFFX-BioB-3_at
452.5
P
0.00004
AFFX-BioC-5_at
1692.7
P
0.00004
AFFX-BioC-3_at
1204.9
P
0.00004
AFFX-BioDn-5_at
3510.5
P
0.00004
AFFX-BioDn-3_at
4998.1
P
0.00004
AFFX-CreX-5_at
13971.9
P
0.00004
AFFX-CreX-3_at
18623
P
0.00004
AFFX-DapX-5_at
1506.7
P
0.00004
AFFX-DapX-M_at
1981.5
P
0.00005
AFFX-DapX-3_at
3360.2
P
0.00004
AFFX-LysX-5_at
153.8
P
0.00004
AFFX-LysX-M_at
209.2
P
0.00007
AFFX-LysX-3_at
461.4
P
0.00005
AFFX-PheX-5_at
298
P
0.00006
AFFX-PheX-M_at
410
P
0.00005
AFFX-PheX-3_at
478.8
P
0.00004
AFFX-ThrX-5_at
421
P
0.00007
AFFX-ThrX-M_at
549.1
P
0.00005
22810 568 Kbytes .Supplementary file Size Download File type/resource GSM88056.CEL.gz
1.9 Mb
(ftp) (http) CEL
Processed data included within Sample table
