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Status
Public on Apr 24, 2008
Title
csn4-1_mutant replicate 2
Sample type
RNA
Source name
7 day old seedlings
Organism
Arabidopsis thaliana
Characteristics
mutant, 7 day old seedlings, light grown
Extracted molecule
total RNA
Extraction protocol
Approximately 80 mg from 7 day old light grown csn4-1 mutant seedlings were collected and ground to a fine powder under liquid Nitrogen. Samples were lysed in separate groups in RLT buffer (Qiagen).Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
Label
biotin
Label protocol
Approximately 6 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
csn4-1 mutant 7 day old seedlings light grown
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 15, 2005
Last update date
Aug 28, 2018
Contact name
Claus Schwechheimer
E-mail(s)
claus.schwechheimer@wzw.tum.de
Organization name
TUM
Department
Plant Systems Biology
Street address
Emil-Ramann-Str. 4
City
Freising
ZIP/Postal code
85354
Country
Germany
Platform ID
GPL198
Series (2)
GSE3865
CSN4-1 mutant analysis
GSE9728
COP9 signalosome (csn) mutant analysis
Relations
Reanalyzed by
GSE119083
Data table header descriptions
ID_REF
VALUE
MAS5-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was P (P), A (A), M (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
3511.9
P
0.00004
AFFX-BioB-M_at
4420
P
0.00004
AFFX-BioB-3_at
2954.9
P
0.00004
AFFX-BioC-5_at
9970.3
P
0.00004
AFFX-BioC-3_at
7214
P
0.00004
AFFX-BioDn-5_at
20518.4
P
0.00004
AFFX-BioDn-3_at
26835.2
P
0.00004
AFFX-CreX-5_at
72242.4
P
0.00004
AFFX-CreX-3_at
101369.4
P
0.00004
AFFX-DapX-5_at
613.7
P
0.00006
AFFX-DapX-M_at
3038.4
P
0.00005
AFFX-DapX-3_at
13394.4
P
0.00004
AFFX-LysX-5_at
310.3
P
0.00066
AFFX-LysX-M_at
854.5
P
0.0002
AFFX-LysX-3_at
4292
P
0.00007
AFFX-PheX-5_at
99
A
0.26283
AFFX-PheX-M_at
450.7
P
0.00141
AFFX-PheX-3_at
3584.8
P
0.00005
AFFX-ThrX-5_at
38.4
A
0.57404
AFFX-ThrX-M_at
236.7
P
0.00086
Total number of rows: 22810 Table truncated, full table size 572 Kbytes .Supplementary file
Size
Download
File type/resource
GSM88050.CEL.gz
1.7 Mb
(ftp) (http)
CEL
Processed data included within Sample table