|
| Status |
Public on Jun 25, 2013 |
| Title |
MCL_IGHV_MUT (R1399_M) |
| Sample type |
RNA |
| |
|
| Source name |
B cell lymphocytes from MCL patient
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Mantle Cell Lymphoma
cell type: B cell lymphocyte
ighv status: MUT
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from mononuclear cell preparations of leukemic B cells obtained fromp peripheral blood prior to treatment using TRIZOL. RNA quality and quantity was assessed using the bionalyzer 2100 and nanodrop spectrophotometer
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol using the 3' IVT Express kit from Affymetrix
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HU133plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
R1399_M.CEL
|
| Data processing |
The data were analyzed with the Expression Console applilcation from Affymetrix. We performed RMA to summarize the data.
|
| |
|
| Submission date |
Feb 22, 2012 |
| Last update date |
Jun 25, 2013 |
| Contact name |
Pedro Jares |
| E-mail(s) |
[email protected]
|
| Organization name |
IDIBAPS
|
| Department |
Genomics Unit
|
| Street address |
c/ Villarroel 170
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
|
