|
| Status |
Public on Jan 09, 2006 |
| Title |
2hr shaded replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
hypocotyl
|
| Organism |
Brassica oleracea |
| Characteristics |
wild type, 3 day old seedling
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Approximately 100 3-day-old B. oleracea seedlings that has been stimulated with unidirectional blue-light were trisected into lit flanks, vasculature, and shaded flanks. The lit and shaded flanks were collected and Total RNA was extracted from the tissue using an RNeasy kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Approximately 20 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
wild type, blue-light treated Brassica hypocotyl tissue
|
| Data processing |
GCOS 3.0
|
| |
|
| Submission date |
Dec 14, 2005 |
| Last update date |
Jan 09, 2006 |
| Contact name |
Emmanuel Liscum |
| E-mail(s) |
[email protected]
|
| Phone |
573-882-2672
|
| Fax |
573-884-9676
|
| URL |
http://www.biosci.missouri.edu/liscum/liscumlabpage.html
|
| Organization name |
University of Missouri
|
| Department |
Biological Sciences
|
| Lab |
Life Sciences Center
|
| Street address |
Rollin Rd
|
| City |
Columbia |
| State/province |
MO |
| ZIP/Postal code |
65211 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE3847 |
Tropic-stimulus induced changes in gene expression |
|
