|
| Status |
Public on Dec 13, 2005 |
| Title |
Microdissected Ulcerative Colitis |
| Sample type |
RNA |
| |
|
| Source name |
Human Ulcerative Colitis cell from formalin-fixed, paraffin-embedded (FFPE) samples
|
| Organism |
Homo sapiens |
| Characteristics |
Tissue: Human Colon
State: Ulcerative Colitis
Gender: 2 male and 1 female pooled
|
| Biomaterial provider |
Asan Medical center
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol reagent(invtrogen, Carlsbad,CA.) . two-cycle cDNA synthesis, assays were performed using two-cycle cDNA synthesis kit (Affymetrix: 900432) according to manufacturer’s instruction.
|
| Label |
Biotin
|
| Label protocol |
cRNA was produced by GeneChip IVT labeling kit (Affymetrix: 900449) according to manufacturer’s instruction.
|
| |
|
| Hybridization protocol |
Fragmented cRNA was hybridized to X3P array at 45℃ for 16 hrs.
|
| Scan protocol |
Scanning of the array was done by Affymetrix GeneChip Scanner3000.
|
| Description |
ulcerative colitis
|
| Data processing |
standard Affymetrix protocol
|
| |
|
| Submission date |
Dec 11, 2005 |
| Last update date |
Dec 19, 2005 |
| Contact name |
Inchul Lee |
| E-mail(s) |
[email protected]
|
| Phone |
82-2-3010-4167
|
| URL |
http://iclee.amc.seoul.kr
|
| Organization name |
University of Ulsan College of Medicine
|
| Department |
Pathology
|
| Lab |
Disease Genomic Research Lab.
|
| Street address |
388-1 Poongnap-Dong, Songpa-Gu
|
| City |
Seoul |
| ZIP/Postal code |
138-736 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL1352 |
| Series (1) |
| GSE3801 |
Gene Expression Profiling of Ulcerative Colitis |
|
