|
| Status |
Public on Apr 01, 2012 |
| Title |
surgical resection HC12 |
| Sample type |
RNA |
| |
|
| Source name |
combined hepatocellular-cholangiocarcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue type: combined hepatocellular-cholangiocarcinoma
gender: male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was extracted from clinical and pathologic records from patient in agreement with French laws and ethical guidelines related to the protection of the patient
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 300 ng total RNA (Following the GeneChip whole transcript (WT) Sense Target labelling assay kit Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cDNA were hybridized for 16 hr at 45C on GeneChip murine Gene from Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from combined hepatocellular-cholangiocarcinoma
|
| Data processing |
Affymetrix expression console was used to check the scaned data and RMA on R programm was used as the normalisation method.
|
| |
|
| Submission date |
Jan 24, 2012 |
| Last update date |
Apr 01, 2012 |
| Contact name |
Florent Dumont |
| E-mail(s) |
[email protected]
|
| Organization name |
Université Paris-Saclay
|
| Street address |
17 avenue des sciences
|
| City |
91400 Orsay |
| State/province |
Ile de France |
| ZIP/Postal code |
91400 |
| Country |
France |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE35306 |
Combined hepatocellular-cholangiocarcinomas exhibit progenitor features and activation of Wnt and TGFbeta signaling pathways |
|
