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Status
Public on Dec 09, 2005
Title
PP6, chip HG-U133A
Sample type
RNA
Source name
Muscle stem cell, Muscle
Organism
Homo sapiens
Characteristics
Strain:
Growth protocol
Growth medium: F10 Media supplements: 10% SVF, 9mM glutamine
Extracted molecule
total RNA
Extraction protocol
RNA prep method: TRIzol Cell purification method: After 24 hours of culture, floating cells were collected and plated on new flasks, serial preplates were obtained until pp6.
Label
Biotin
Description
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/ ). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/ ) by Dr. Jacques P. Tremblay ([email protected] ; 2705 boulevard Laurier) for analysis. Stembase Experiment ID: E28 Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=28 SCGP Sample ID: S31 SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=28#SAMPLE_29 Short description: PP6: human muscle derived stem cell RNA concentration: 0.9 ug/ul Sample volume: 8.0 ul
Data processing
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
Submission date
Dec 07, 2005
Last update date
Jan 19, 2006
Organization
Ottawa Hospital Research Institute
Phone
(613) 737-8899 -73255
Department
Cellular and Molecular Medicine
Lab
Ottawa Bioinformatics Core Facility
Street address
501 Smyth Rd.
City
Ottawa
State/province
ON
ZIP/Postal code
K1H 8L6
Country
Canada
Platform ID
GPL96
Series (1)
GSE3769
Comparison of muscle stem cell preplates and myoblasts.
Data table header descriptions
ID_REF
VALUE
'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06.
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-5_at
197.495
P
0.000195116
AFFX-BioB-M_at
504.176
P
6.02111e-05
AFFX-BioB-3_at
339.34
P
4.42873e-05
AFFX-BioC-5_at
484.961
P
6.02111e-05
AFFX-BioC-3_at
424.608
P
4.42873e-05
AFFX-BioDn-5_at
586.145
P
4.42873e-05
AFFX-BioDn-3_at
2856.12
P
5.16732e-05
AFFX-CreX-5_at
4799.9
P
4.42873e-05
AFFX-CreX-3_at
6515.61
P
4.42873e-05
AFFX-DapX-5_at
10.9516
A
0.0956669
AFFX-DapX-M_at
13.9407
A
0.102165
AFFX-DapX-3_at
0.983908
A
0.98333
AFFX-LysX-5_at
4.46323
A
0.760937
AFFX-LysX-M_at
1.87425
A
0.794268
AFFX-LysX-3_at
1.18944
A
0.876428
AFFX-PheX-5_at
1.18859
A
0.945787
AFFX-PheX-M_at
7.291
A
0.617401
AFFX-PheX-3_at
4.54158
A
0.368438
AFFX-ThrX-5_at
2.36873
A
0.963431
AFFX-ThrX-M_at
2.11404
A
0.772364
Total number of rows: 22283 Table truncated, full table size 657 Kbytes .Supplementary file
Size
Download
File type/resource
GSM86503.CEL.gz
3.2 Mb
(ftp) (http)
CEL
GSM86503.EXP.gz
516 b
(ftp) (http)
EXP