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Sample GSM86503 Query DataSets for GSM86503
Status Public on Dec 09, 2005
Title PP6, chip HG-U133A
Sample type RNA
 
Source name Muscle stem cell, Muscle
Organism Homo sapiens
Characteristics Strain:
Growth protocol Growth medium: F10
Media supplements: 10% SVF, 9mM glutamine
Extracted molecule total RNA
Extraction protocol RNA prep method: TRIzol
Cell purification method: After 24 hours of culture, floating cells were collected and plated on new flasks, serial preplates were obtained until pp6.
Label Biotin
 
Description This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Jacques P. Tremblay ([email protected]; 2705 boulevard Laurier) for analysis.
Stembase Experiment ID: E28
Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=28
SCGP Sample ID: S31
SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=28#SAMPLE_29
Short description: PP6: human muscle derived stem cell
RNA concentration: 0.9 ug/ul
Sample volume: 8.0 ul
Data processing Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
 
Submission date Dec 07, 2005
Last update date Jan 19, 2006
Organization Ottawa Hospital Research Institute
Phone (613) 737-8899 -73255
Department Cellular and Molecular Medicine
Lab Ottawa Bioinformatics Core Facility
Street address 501 Smyth Rd.
City Ottawa
State/province ON
ZIP/Postal code K1H 8L6
Country Canada
 
Platform ID GPL96
Series (1)
GSE3769 Comparison of muscle stem cell preplates and myoblasts.

Data table header descriptions
ID_REF
VALUE 'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06.
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 197.495 P 0.000195116
AFFX-BioB-M_at 504.176 P 6.02111e-05
AFFX-BioB-3_at 339.34 P 4.42873e-05
AFFX-BioC-5_at 484.961 P 6.02111e-05
AFFX-BioC-3_at 424.608 P 4.42873e-05
AFFX-BioDn-5_at 586.145 P 4.42873e-05
AFFX-BioDn-3_at 2856.12 P 5.16732e-05
AFFX-CreX-5_at 4799.9 P 4.42873e-05
AFFX-CreX-3_at 6515.61 P 4.42873e-05
AFFX-DapX-5_at 10.9516 A 0.0956669
AFFX-DapX-M_at 13.9407 A 0.102165
AFFX-DapX-3_at 0.983908 A 0.98333
AFFX-LysX-5_at 4.46323 A 0.760937
AFFX-LysX-M_at 1.87425 A 0.794268
AFFX-LysX-3_at 1.18944 A 0.876428
AFFX-PheX-5_at 1.18859 A 0.945787
AFFX-PheX-M_at 7.291 A 0.617401
AFFX-PheX-3_at 4.54158 A 0.368438
AFFX-ThrX-5_at 2.36873 A 0.963431
AFFX-ThrX-M_at 2.11404 A 0.772364

Total number of rows: 22283

Table truncated, full table size 657 Kbytes.




Supplementary file Size Download File type/resource
GSM86503.CEL.gz 3.2 Mb (ftp)(http) CEL
GSM86503.EXP.gz 516 b (ftp)(http) EXP

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