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Sample GSM864205 Query DataSets for GSM864205
Status Public on Feb 01, 2012
Title HEK293-HIPK2-Vector-sample2
Sample type RNA
 
Source name HEK293 transfected with HIPK2 vector biological rep2
Organism Homo sapiens
Characteristics cell line: HEK293
transfection: HIPK2 vector
gender: NA
Growth protocol Mice were sacrificed at 8 weeks. Each sample in the Tg and WT mice are from different individual animals. One mice developed significant scarring and since the array results were significantly different this sample was not used for further analysis. HEK 293 and HK2 cells were obtained from ATCC and cultured as described in Rosenstiel, P.E., et al. Kidney Int 74, 1049-1058 (2008).
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from kidney cortices of five Tg26 and five WT for gene expression microarrays. RNA was isolated by using RNAesay kit from Qiagen.
Label biotin
Label protocol Following standard procedures according to the manufacturer's instructions as applied at the MSSM core facility.
 
Hybridization protocol Following standard procedures according to the manufacturer's instructions as applied at the MSSM core facility.
Scan protocol Following standard procedures according to the manufacturer's instructions as applied at the MSSM core facility.
Description Mouse samples are labeled as investigator, id of sample, condition, gender, date. Human cellline samples are labled as facility, date, investigator, condition, sample id.
Gene expression from HEK293 transfected with HIPK2 vector biological rep2
Data processing Expression Console (1.1.2800.29167), affymetrix-algorithm-name = rma
One-way analysis of variance test (ANOVA) with BH correction at 0.05 FDR was applied to the dataset to identify the genes that were differentially expressed between groups.
 
Submission date Jan 20, 2012
Last update date Feb 01, 2012
Contact name Avi Ma'ayan
E-mail(s) [email protected]
Phone 2126591739
Organization name Mount Sinai School of Medicine
Department Pharmacology and Systems Therapeutics
Lab Ma'ayan Lab
Street address One Gustave L. Levy Place Box 1215
City New York
State/province NY
ZIP/Postal code 10037
Country USA
 
Platform ID GPL6244
Series (1)
GSE35226 Systems approach identifies HIPK2 as a critical regulator of kidney tubulointerstitial fibrosis

Data table header descriptions
ID_REF
VALUE log2 rma

Data table
ID_REF VALUE
7892501 8.24024
7892502 3.04958
7892503 3.8524
7892504 8.5412
7892505 3.5574
7892506 4.39446
7892507 4.14462
7892508 4.66165
7892509 11.8534
7892510 3.66332
7892511 4.40851
7892512 6.68758
7892513 3.34559
7892514 10.7932
7892515 10.0668
7892516 7.82505
7892517 4.98822
7892518 2.68945
7892519 5.93197
7892520 8.59377

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM864205_MSSM021011JH1DN10Mu2.CEL.gz 4.3 Mb (ftp)(http) CEL
GSM864205_MSSM021011JH1DN10Mu2.CHP.gz 253.8 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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