|
| Status |
Public on Jan 18, 2012 |
| Title |
THP1 |
| Sample type |
RNA |
| |
|
| Source name |
AML
|
| Organism |
Homo sapiens |
| Characteristics |
expression: EVI1 low AML
cell line: THP1
|
| Growth protocol |
Acute myloid leukemia cell lines were cultured in RPMI 1640 medium (Wako) supplemented with 10% fetal bovine serum (UCSD/AML1 : add 1 ng/ml granulocyte-macrophage colony-stimulating factor)..
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
the cRNA was linearly amplified with T7 polymerase, the biotinylated cRNA was cleaned with an RNeasy Mini Column Kit, fragmented to 50 to 200 nucleotides, and then hybridized to the Affymetrix Human Genome U133 Plus 2.0 Array.
|
| Scan protocol |
GeneChips were scanned using Affymetrics GeneChip Scanner 3000.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jan 17, 2012 |
| Last update date |
Jan 18, 2012 |
| Contact name |
Yusuke Saito |
| Organization name |
Miyazaki University
|
| Street address |
5200 Kihara, Kiyotake
|
| City |
Miyazaki |
| ZIP/Postal code |
889-1692 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE35159 |
The expression profiles of AML cell lines |
|
