|
| Status |
Public on Jun 21, 2012 |
| Title |
Wild type biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
S. aureus wt_late exponential growth phase
|
| Organism |
Staphylococcus aureus |
| Characteristics |
background strain: NewmanHG
genotype/variation: wild type
growth phase: late exponential phase
|
| Treatment protocol |
5 ml of cultures were harvested by centrifugation
|
| Growth protocol |
S. aureus strains were grown in complex medium. Bacteria from an overnight culture were diluted to an initial optical density at 600 nm (OD600) of 0.05 in fresh medium and grown with shaking (220 rpm) at 37°C till late exponential growth phase (OD600 = 1.5).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Bacteria were lysed in Trizol reagent (Invitrogen) with 0.5 ml of zirconia-silica beads (0.1-mm diameter) in a high-speed homogenizer. RNA was then isolated as described in the instructions provided by the manufacturer of Trizol.
|
| Label |
biotin-streptavidin
|
| Label protocol |
Standard affymetrix labelling protocol for prokaryotic organism
|
| |
|
| Hybridization protocol |
Standard affymetrix hybridization protocol for GeneChip S. aureus Genome Array
|
| Scan protocol |
Standard scanning protocol for affymetrix GeneChip Scanner 3000 7G system
|
| Description |
Gene expression data from S. aureus NewmanHG wild type at late exponential growth phase
|
| Data processing |
GC-RMA normalization and t-test statistics was performed with GenespringGX 11.5.1 software.
|
| |
|
| Submission date |
Jan 10, 2012 |
| Last update date |
Jun 21, 2012 |
| Contact name |
Gabriella Marincola |
| Organization name |
IMIT - Interfaculty Institute for Microbiology and Infection Medicine
|
| Street address |
Elfriede Auhlornstr. 6
|
| City |
72076 |
| ZIP/Postal code |
Tübingen |
| Country |
Germany |
| |
|
| Platform ID |
GPL1339 |
| Series (1) |
| GSE34980 |
RNase Y of Staphylococcus aureus and its role in the activation of virulence genes |
|
