|
| Status |
Public on Jan 30, 2012 |
| Title |
GFP-positive cells from twi-GAL4 UAS-2EGFP embryos, replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
FACS-sorted Drosophila cells
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell type: FACS-sorted GFP positive cells
cell derivation: single cell suspensions of late stage 11/early stage 12 embryos
genotype/variation: twi-GAL4 and UAS-2EGFP insertions that target GFP fluorescence to Twist expressing mesodermal cells
|
| Growth protocol |
Embryos were aged to late stage 11-early stage 12 at 29°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions of sorted mesodermal cells.
|
| Label |
biotin
|
| Label protocol |
Approximately 2-5 µg of total RNA was processed to produce biotinylated cRNA targets
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
FACS-sorted GFP positive cells from single cell suspensions of late stage 11/early stage 12 embryos with twi-GAL4 and UAS-2EGFP insertions that target GFP fluorescence to Twist expressing mesodermal cells.
|
| Data processing |
The data were analyzed with the Bioconductor affy package, using the mas5 command with default settings, and log2 transformed.
|
| |
|
| Submission date |
Jan 09, 2012 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Shaad M. Ahmad |
| E-mail(s) |
[email protected]
|
| Organization name |
National Heart Lung and Blood Institute
|
| Department |
Systems Biology Center
|
| Lab |
Laboratory of Developmental Systems Biology
|
| Street address |
Building 10, Room 7N311, 10 Center Drive MSC 1654
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE34946 |
Two Forkhead transcription factors regulate the division of cardiac progenitor cells by a Polo-dependent pathway - II |
|
| Relations |
| Reanalyzed by |
GSE119084 |
