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Sample GSM8583617

Query DataSets for GSM8583617

Status

Public on Jan 11, 2025

Title

Monascus purpureus mycelia under optimized metal ion, Sample 1, Day1

Sample type

SRA

Source name

mycelia

Organism

Monascus purpureus

Characteristics

tissue: mycelia
cell line: Monascus purpureus MP2022
genotype: WT
treatment: Treated with 0.0 g/L ZnSO4·2H2O and 2.5 g/L MgSO4·2H2O
time point: Day1

Extracted molecule

total RNA

Extraction protocol

RNA was harvested using TRlzol Reagent (Life technologies, California, USA). 0.1-1.0 μg of total RNA was used for the construction of sequencing libraries.
The library was constructed using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, E7530) and NEBNext Multiplex Oligos for Illumina (NEB, E7500).
In briefly, the enriched mRNA was fragmented into approximately 200 nt RNA inserts, which were used to synthesize the first-strand cDNA and the second cDNA. The double-stranded cDNA were performed end-repair/dA-tail and adaptor ligation. The suitable fragments were isolated by Agencourt AMPure XP beads (Beckman Coulter, Inc.), and enriched by PCR amplification. Finally, the constructed cDNA libraries of M. purpureus were sequenced on a flow cell using an Illumina HiSeq™ sequencing platform.

Library strategy

RNA-Seq

Library source

transcriptomic

Library selection

cDNA

Instrument model

Illumina HiSeq 2000

Description

Data processing

The clean reads that were filtered from the raw reads were mapped to M. purpureus genome using Tophat2 software.
Assembly: ASM2599979v1
Supplementary files format and content: FPKM values for each sample

Submission date

Oct 21, 2024

Last update date

Jan 11, 2025

Contact name

Xia Yi

E-mail(s)

[email protected]

Organization name

Changzhou University

Street address

Gehu Middle Road #21