|
| Status |
Public on Jan 07, 2012 |
| Title |
G9a knockdown cells, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
MDA-MB231 cells, G9a knockdown
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB231
treatment: G9a knockdown
cell type: less metastatic breast cancer cells
|
| Growth protocol |
Cells were grown in DMEM/F12 plus 10%FBS in 5%CO2 at 37C. Cells were trypsinized and harvested when in log-growth phase.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells using the Qiagen RNA purification kit following the manfacturer's protocol.
|
| Label |
Biotin
|
| Label protocol |
Labeling was performed at the core technician at the microarry core facility at the University of Kentucky School of Medicine.
|
| |
|
| Hybridization protocol |
Hybridization was performed at the core technician at the microarry core facility at the University of Kentucky School of Medicine.
|
| Scan protocol |
Scanning was performed at the core technician at the microarry core facility at the University of Kentucky School of Medicine.
|
| Description |
Gene expression array of less metastatic breast tumor cell line.
|
| Data processing |
Data processing was performed at the core technician at the microarry core facility at the University of Kentucky School of Medicine.
|
| |
|
| Submission date |
Jan 06, 2012 |
| Last update date |
Jan 07, 2012 |
| Contact name |
Binhua P Zhou |
| E-mail(s) |
[email protected]
|
| Phone |
859-323-4474
|
| Organization name |
University of Kentucky School of Medicine
|
| Department |
Markey Cancer Center/Biochemistry
|
| Lab |
Zhou's lab
|
| Street address |
789 South Limestone
|
| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40536 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE34925 |
Expression data from knockdown of G9a in MDA-MB231 cells |
|
