|
| Status |
Public on Jan 07, 2012 |
| Title |
BM_CMP_KO_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
tissue: bone marrow
strain: C57BL/6
genotype: IRF-8 knockout
cell type: common myeloid progenitors (Lineage-c-kithiSca-1-CD11c- CD34+ Flk2+CD16/32-CD115-)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Qiagen Rneasy Kit per manufacturer's instructions. RNA quality was accessed using the Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated aRNA was prepared using the Nugen pico-amplification kit per manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
data analyzed using ArrayStar 4 (DNAstar) using quantiles-based normalization
|
| |
|
| Submission date |
Jan 06, 2012 |
| Last update date |
Aug 02, 2013 |
| Contact name |
Amy M. Becker |
| E-mail(s) |
[email protected]
|
| Phone |
314-362-8782
|
| Fax |
314-362-8888
|
| Organization name |
Washington University School of Medicine
|
| Department |
Pathology and Immunology
|
| Lab |
Deepta Bhattacharya
|
| Street address |
660 S. Euclide Ave.
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL15097 |
| Series (2) |
| GSE34915 |
IRF-8 extinguishes neutrophil production and promotes dendritic cell lineage commitment in both myeloid and lymphoid progenitors (Illumina). |
| GSE34917 |
IRF-8 extinguishes neutrophil production and promotes dendritic cell lineage commitment in both myeloid and lymphoid progenitors |
|
