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Sample GSM857305 Query DataSets for GSM857305
Status Public on Apr 26, 2012
Title 24h E.coli-infected, control IgG-treated lungs, biological rep1
Sample type RNA
 
Source name 24h_E.coli-infection + control IgG-treated lungs
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: Lung
infection: intratracheal instillation of E. coli (10^6 CFU)
antibody included in the instillate: Control IgG
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions (Life Technologies). RNA was further purified using the the Qiagen RNEasy kit with the optional DNAse treatment.
Label Biotin
Label protocol Fragmented, biotinlyated sense strand DNA were prepared from 200 ng total RNA using the WT Target labeling kit according to the manufacturer's protocol (Affymetrix)
 
Hybridization protocol 5 ug of labeled sense strand DNA were hybridized for 17h at 45 degrees C on a Genechip Mouse Gene Array 1.0 ST Array. GeneChips were washed and stainied in the Affymetrix Fluiidix Station 450.
Scan protocol Arrays were scanned using the Affymetrix GeneChip Scanner 3000 7GPlus.
Description Ecoli_IgG1
Data processing CEL files were summarized using the Affymetrix Expression Console (version 1.1). The RMA (Robust Multi-Array Analysis) algorithm was used to generate gene-level data.
 
Submission date Jan 06, 2012
Last update date Apr 26, 2012
Contact name Lee Quinton
Organization name Boston University School of Medicine
Street address 72 E Concord St
City Boston
State/province MA
ZIP/Postal code 02118
Country USA
 
Platform ID GPL11078
Series (1)
GSE34901 Expression data from mouse lungs during E. coli pneumonia in the presence or absence of LIF neutralization

Data table header descriptions
ID_REF
VALUE Log2 RMA data

Data table
ID_REF VALUE
100009600_at 3.17
100009609_at 2.28
100012_at 2.64
100017_at 7.42
100019_at 5.21
100033459_at 6.56
100034251_at 10.55
100036521_at 5.86
100037258_at 8.74
100037278_at 4.76
100038380_at 4.66
100038429_at 4.02
100038486_at 3.03
100038570_at 4.58
100038598_at 4.16
100038600_at 4.27
100038618_at 4.19
100038635_at 5.49
100038680_at 3.38
100038683_at 4.27

Total number of rows: 21212

Table truncated, full table size 300 Kbytes.




Supplementary file Size Download File type/resource
GSM857305_Ecoli_IgG1.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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