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Status |
Public on Apr 26, 2012 |
Title |
24h E.coli-infected, control IgG-treated lungs, biological rep1 |
Sample type |
RNA |
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Source name |
24h_E.coli-infection + control IgG-treated lungs
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Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 tissue: Lung infection: intratracheal instillation of E. coli (10^6 CFU) antibody included in the instillate: Control IgG
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions (Life Technologies). RNA was further purified using the the Qiagen RNEasy kit with the optional DNAse treatment.
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Label |
Biotin
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Label protocol |
Fragmented, biotinlyated sense strand DNA were prepared from 200 ng total RNA using the WT Target labeling kit according to the manufacturer's protocol (Affymetrix)
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Hybridization protocol |
5 ug of labeled sense strand DNA were hybridized for 17h at 45 degrees C on a Genechip Mouse Gene Array 1.0 ST Array. GeneChips were washed and stainied in the Affymetrix Fluiidix Station 450.
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Scan protocol |
Arrays were scanned using the Affymetrix GeneChip Scanner 3000 7GPlus.
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Description |
Ecoli_IgG1
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Data processing |
CEL files were summarized using the Affymetrix Expression Console (version 1.1). The RMA (Robust Multi-Array Analysis) algorithm was used to generate gene-level data.
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Submission date |
Jan 06, 2012 |
Last update date |
Apr 26, 2012 |
Contact name |
Lee Quinton |
Organization name |
Boston University School of Medicine
|
Street address |
72 E Concord St
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02118 |
Country |
USA |
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Platform ID |
GPL11078 |
Series (1) |
GSE34901 |
Expression data from mouse lungs during E. coli pneumonia in the presence or absence of LIF neutralization |
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