|
| Status |
Public on Dec 16, 2011 |
| Title |
TP25-2 |
| Sample type |
genomic |
| |
|
| Source name |
Blood DNA
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
age: 70
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from peripheral blood with QIAGEN kits
|
| Label |
C-Bio and A-DNP
|
| Label protocol |
500ng of genomic DNA was whole-genome amplified in an overnight reaction at 37¡C using amplification master mix (WG-AMM) and primer/neutralization mix (WG-MP1). After incubation the amplified DNA was fragmented with fragmentation mix (WG-FRG), precipitated with isopropanol and precipitation mix (PA1) and resuspended in hybridization buffer (RA1).
|
| |
|
| Hybridization protocol |
RA1 resuspended DNA was loaded onto BeadChips arrays. After overnight incubation at 48¡C, single-base extension and allele-specific staining was performed on a Teflow chamber rack system (Tecan, Maennedorf, Switzerland).
|
| Scan protocol |
After allele-specific staining BeadChip arrays were coated with XC4/ethanol , dried for 1 hour and scanned on a BeadArray Reader (Illumina).
|
| Description |
Genomic DNA extracted from blood was genotyped using Infinium Human660W Quad Genotyping BeadChips (Illumina).
|
| Data processing |
Genotypes and other intensity data were called and exported with Illumina GenomeStudio software
|
| |
|
| Submission date |
Dec 15, 2011 |
| Last update date |
Dec 19, 2011 |
| Contact name |
Devin Absher |
| E-mail(s) |
[email protected]
|
| Organization name |
HudsonAlpha Institute for Biotechnology
|
| Street address |
601 Genome Way
|
| City |
Huntsville |
| State/province |
AL |
| ZIP/Postal code |
35806 |
| Country |
USA |
| |
|
| Platform ID |
GPL8888 |
| Series (1) |
| GSE34467 |
Somatic copy number variants in monozygotic twins and elderly single-born individuals |
|
