|
| Status |
Public on Jan 23, 2012 |
| Title |
wild-type replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
splenic CD11c+ dendritic cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: splenic CD11c+ dendritic cells
strain: C57Bl/6strain: C57Bl/6
genotype/variation: WT
treatment: tamoxifen
|
| Treatment protocol |
CD11c+ dendritic cells were purified from the spleen by flow cytometry and then lyzed in RNA extraction buffer.
|
| Growth protocol |
Wild-type and Map3k7-CreER mice were treated with tamoxifen in vivo for 3 days to induce deletion of Map3k7 (TAK1).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Qiagen RNeasy micro kit according to the manufacturer's recommended protocol.
|
| Label |
biotin
|
| Label protocol |
100 ng of total RNA was converted to biotin-labeled cRNA using the Affymetrix 3'-IVT Express protocol as per the manufacturer's recommendations
|
| |
|
| Hybridization protocol |
5 micrograms of biotin-labeled cRNA was hybridized to an Affymetrix HT_MG-430_PM array using the Affymetrix GeneTitan system
|
| Scan protocol |
Arrays were scanned using the Affymetrix GeneTitan system
|
| Data processing |
Signals on arrays were summarized using the RMA method as implemented in the Affymetrix Expression Console software v1.1
|
| |
|
| Submission date |
Dec 13, 2011 |
| Last update date |
Jan 23, 2012 |
| Contact name |
Geoffrey Neale |
| E-mail(s) |
[email protected]
|
| Organization name |
St Jude Childrens Research Hospital
|
| Department |
Hartwell Center
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE34417 |
TAK1-dependent checkpoint in dendritic cell survival regulates immune homeostasis and function |
|
