|
| Status |
Public on Sep 15, 2012 |
| Title |
Colo205RC PLX4720 resistant cell line |
| Sample type |
RNA |
| |
|
| Source name |
Colo205RC PLX4720 resistant cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Colo205RC PLX4720 resistant cell line
cell type: colon cancer
|
| Treatment protocol |
Resistant lines were grown in PLX4720-free media x 24 hours prior to harvesting
|
| Growth protocol |
Culture dish + Media with 10%serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted cell lines using the Qiagen RNeasy Kit according to the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
hybridization to Affymetrix HGU133 plus2 according to the standard Affymetrix protocol
|
| Scan protocol |
imaging on GeneChip Scanner 3000 (Affymetrix)
|
| Data processing |
The data were first quantified using the MAS5 algorithm. The Robust Multiarray Analysis (RMA) algorithm was used to normalize and quantify the data.
|
| |
|
| Submission date |
Dec 09, 2011 |
| Last update date |
Sep 15, 2012 |
| Contact name |
Scott Kopetz |
| E-mail(s) |
[email protected]
|
| Phone |
713-792-2828
|
| Organization name |
MD Anderson Cancer Center
|
| Street address |
1515 Holcombe Blvd, Unit 426
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE34299 |
Expression of BRAF inhibitor resistant colon cancer lines |
|
