|
| Status |
Public on Jan 19, 2013 |
| Title |
Pi_adult_mi_RNA_pool_c |
| Sample type |
RNA |
| |
|
| Source name |
Musculus longissimus dorsi tissue from adult pig taken at slaughter
|
| Organism |
Sus scrofa |
| Characteristics |
breed: Pietrain
developmental stage: adult
tissue type: skeletal muscle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Qiagen miReasy Mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol for small-RNA. Then small RNA was cleaned up using the Rneasy MinElute Clean up kit (Qiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from miRNA according to FlashTag™ Biotin RNA Labeling Kit for Affymetrix GeneChip miRNA arrays (Genisphere, Hatfield, PA, USA) according to the manufacturer’s recommendations Affymetrix® GeneChip® miRNA Array protocols (Affymetrix, Santa Clara, CA, USA)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 42C on Affymetrix GeneChip miRNA arrays. GeneChips were washed and processed to detect biotin-containing transcripts by Streptavidin-PE (Phycoerythrin) conjugate.
|
| Scan protocol |
Scanning using the GeneChip scanner 3000 were performed according to Affymetrix protocols
|
| Description |
Gene expression data from skeletal muscle of adult pig breed (Pi).
|
| Data processing |
Data were analysed with Affymetrix GCOS 1.1.1 software using global scaling to a target signal of 500.
|
| |
|
| Submission date |
Dec 07, 2011 |
| Last update date |
Jan 19, 2013 |
| Contact name |
Klaus Wimmers |
| E-mail(s) |
[email protected]
|
| Phone |
0049 3820868700
|
| Organization name |
Leibniz Institute for Farm Animal Biology (FBN)
|
| Street address |
Wilhelm-Stahl-Allee 2
|
| City |
Dummerstorf |
| ZIP/Postal code |
18196 |
| Country |
Germany |
| |
|
| Platform ID |
GPL14969 |
| Series (1) |
| GSE34213 |
Gene expression data of micro RNA from skeletal muscle sample of pig DL and Pi breed |
|
