|
| Status |
Public on Dec 08, 2011 |
| Title |
spxA1-null mutant of Streptococcus sanguinis mixed with wild type SK36, replicate 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Streptococcus sanguinis wild type
|
| Organism |
Streptococcus sanguinis |
| Characteristics |
strain: SK36
genotype: wild type
|
| Growth protocol |
S. sangunis SK36 was grown in brain heart infusion broth (BHI; Difco Inc., Detroit, MI) under microaerobic conditions at 37 °C. Overnight culture was diluted into fresh BHI broth by 1% inoculation and cultured further for 5 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy mini kit (Qiagen) following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Microbial RNA Aminoallyl Labeling was performed following the protocol at http://pfgrc.jcvi.org/index.php/microarray/protocols.html.
|
| |
|
| Channel 2 |
| Source name |
Streptococcus sanguinis ΔspxA1
|
| Organism |
Streptococcus sanguinis |
| Characteristics |
strain: SK36
genotype: ΔspxA1
|
| Growth protocol |
S. sangunis ΔspxA1 was grown in brain heart infusion broth (BHI; Difco Inc., Detroit, MI) under microaerobic conditions at 37 °C. Overnight culture was diluted into fresh BHI broth by 1% inoculation and cultured further for 5 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy mini kit (Qiagen) following manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Microbial RNA Aminoallyl Labeling was performed following the protocol at http://pfgrc.jcvi.org/index.php/microarray/protocols.html.
|
| |
|
| |
| Hybridization protocol |
Hybridization at 42C for 16-20 hrs was performed following the protocol at http://pfgrc.jcvi.org/index.php/microarray/protocols.html.
|
| Scan protocol |
Scanned on a GenePix 4000A scanner.
Images were analyzed using the program Spotfinder (v321win).
|
| Description |
Biological replicate 2 of RNA mixture from Streptococcus sanguinis ΔspxA1 and the wild type SK36.
|
| Data processing |
Data were LOWESS normalized and background subtracted using Midas (v2.20). Processed Red signal/processed Green signal ratios were calculated using Excel.
|
| |
|
| Submission date |
Dec 06, 2011 |
| Last update date |
Dec 08, 2011 |
| Contact name |
Lei Chen |
| E-mail(s) |
[email protected]
|
| Phone |
86-22-27406364
|
| Organization name |
Tianjin University
|
| Street address |
92 Weijin Rd
|
| City |
Tianjin |
| ZIP/Postal code |
300072 |
| Country |
China |
| |
|
| Platform ID |
GPL14967 |
| Series (1) |
| GSE34203 |
spxA1-null mutant of Streptococcus sanguinis SK36 |
|
