|
| Status |
Public on Mar 27, 2012 |
| Title |
TS543_72h_miR34a_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
TS543 GBM cells, 72h transfection with hsa-miR-34a
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: TS543 proneural GBM
treatment: 72h post transfection with hsa-miR-34a
|
| Treatment protocol |
TS543 cells were transfected with 100 nM hsa-miR-34a using Hiperfect transfection reagent (Qiagen) for 72h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using mirVana miRNA isolation kit (Ambion) and then assayed using the Agilent Systems Bioanalyzer 2100 to ensure that high-quality RNA was used for the array experiments.
|
| Label |
Cy3
|
| Label protocol |
standard as recommended by illumina
|
| |
|
| Hybridization protocol |
standard as recommended by illumina
|
| Scan protocol |
standard as recommended by illumina
|
| Description |
standard as recommended by illumina
|
| Data processing |
The microarray dataset was normalized using a variance stable normalization (VSN) procedure in the lumi package from the Bioconductor framework.
|
| |
|
| Submission date |
Dec 02, 2011 |
| Last update date |
Mar 27, 2012 |
| Contact name |
Joachim Silber |
| Organization name |
Memorial Sloan-Kettering Cancer Center
|
| Street address |
1275 York Ave #20
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE34242 |
miR-34a targets PDGFRA in proneural malignant glioma |
|
