|
| Status |
Public on Jul 23, 2012 |
| Title |
CHO 12h PHx |
| Sample type |
RNA |
| |
|
| Source name |
pair-fed liver, 12h after PHx
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: liver
diet: carbohydrate
partial hepatectomy time point: t=12h
animal identifiers within pooled samples: H-243,H-264,H-279,H-291
time post surgery: 12h
treatment: after hepatectomy
|
| Treatment protocol |
Liver tissue was freeze-clamped in liquid nitrogen at the time of partial hepatectomy (PHx) and 1 h, 6 h, 12 h, and 24 h after PHx. Tissue was then stored at -80 degrees C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
RNA was labeled with Agilent's miRNA Complete Labeling and Hybridization Kit, according to the manufacturer's instructions
|
| |
|
| Hybridization protocol |
Hybrdization was performed according to the manufacturer's instructions as given in Agilent's miRNA Complete Labeling and Hybridization Kit
|
| Scan protocol |
Slides were scanned on an Agilent DNA Microarray Scanner (G2539A, Agilent) using the recommended settings for miRNA microarrays
|
| Description |
miRNA expression in pair-fed carbohydrate control liver 12h after PHx
|
| Data processing |
miRNA microarray data were extracted with Agilent Feature Extraction Software 10.7.3.1 (Agilent) and quantile normalized with GeneSpring GX version 11.0.2 (Agilent). An EtOH LLM sample found to be a technical outlier was not used in further analysis and was replaced in analysis with the mean of the three remaining EtOH LLM samples.
|
| |
|
| Submission date |
Nov 30, 2011 |
| Last update date |
Jul 23, 2012 |
| Contact name |
Rachael Dippold |
| Organization name |
Thomas Jefferson University
|
| Department |
Pathology, Anatomy, and Cell Biology
|
| Street address |
1020 Locust St., JAH 222
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
| |
|
| Platform ID |
GPL14889 |
| Series (1) |
| GSE34057 |
miRNA expression during liver regeneration in the chronic ethanol-fed rat |
|
