|
| Status |
Public on Aug 01, 2024 |
| Title |
Eye-antennal disc, RNAseq scrib fos mutant Replicate 1 |
| Sample type |
SRA |
| |
|
| Source name |
Eye-antennal disc
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: Eye-antennal disc
genotype: eyFLP FRT82B Cell lethal / FRT82B scrib1 fosKM
|
| Growth protocol |
Flies were reared at 25°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were pelleted and resuspended in Trizol. RNA was isolated according to manufacturer’s protocol. Samples from sorts executed on different days were pooled into three different samples containing 180-330ng RNA.
Libraries prepared according to standard protocols (TruSeq RNA, Illumina).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
KAYSCRIB1
|
| Data processing |
RNA alignment: subread and featureCounts
RNA analysis: edgeR to compute RPKM
Assembly: dm6
Supplementary files format and content: RNAseq_count.table.csv contains list of all genes with the raw counts from the replicates
|
| |
|
| Submission date |
Jul 11, 2024 |
| Last update date |
Aug 01, 2024 |
| Contact name |
Kieran Harvey |
| E-mail(s) |
[email protected]
|
| Organization name |
Peter MacCallum Cancer Centre
|
| Department |
Research
|
| Lab |
Kieran Harvey
|
| Street address |
305 Grattan Street
|
| City |
Melbourne |
| State/province |
Victoria |
| ZIP/Postal code |
3000 |
| Country |
Australia |
| |
|
| Platform ID |
GPL19132 |
| Series (1) |
| GSE272049 |
The JNK and Hippo pathways control epithelial integrity and prevent tumour initiation by regulating an overlapping transcriptome [RNA-Seq] |
|
| Relations |
| BioSample |
SAMN42437583 |
| SRA |
SRX25288576 |
