|
| Status |
Public on Sep 06, 2012 |
| Title |
polymorphonuclear leukocyte cells treated with iPVL time 30 replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
polymorphonuclear leukocyte cells treated with iPVL time 30 min
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: polymorphonuclear leukocyte
|
| Treatment protocol |
PMNs (1 × 107) in RPMI/H were cultured with 1 nM PVL or iPVL at 37°C for 30, 60, or 180 min.
|
| Growth protocol |
PMNs were isolated from venous whole blood of healthy individuals as described [PMID:11983860]. Each subject gave informed consent prior to participation in the study and all work was approved by the Institutional Review Board for Human Subjects, National Institute of Allergy and Infectious Diseases, National Institutes of Health, USA.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
At each indicated time point, PMNs were lysed with RLT buffer and RNA was purified
|
| Label |
biotin
|
| Label protocol |
12 µg of biotin-labeled cRNA target as previously described [PMID:11983860]
|
| |
|
| Hybridization protocol |
Samples from 3 different PMN donors were hybridized on HU133+2 GeneChips (Affymetrix, Santa Clara, CA) according to manufacturer's recommendations
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7GPlus.
|
| Description |
3605_SFG_1_human_23_Denat PVL-30-2_01_5731
Gene expression data from PMN leukocyte cells treated with iPVL time 30 replicate 2
|
| Data processing |
The data were analyzed with GCOS 1.4 using Affymetrix default analysis. The trimmed mean target intensity of each array was arbitrarily set to 1250.
|
| |
|
| Submission date |
Nov 23, 2011 |
| Last update date |
Sep 06, 2012 |
| Contact name |
Dan Sturdevant |
| E-mail(s) |
[email protected]
|
| Phone |
4063639248
|
| Organization name |
NIH
|
| Department |
NIAID
|
| Lab |
RTS
|
| Street address |
903 S 4th street
|
| City |
Hamilton |
| State/province |
MT |
| ZIP/Postal code |
59840 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE33939 |
Sublytic concentrations of Staphylococcus aureus Panton-Valentine leukocidin alter human polymorphonuclear leukocyte gene expression and enhance bactericidal capacity. |
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