|
| Status |
Public on Jul 05, 2012 |
| Title |
soleus of wt Y mouse F4-4 |
| Sample type |
RNA |
| |
|
| Source name |
soleus of wt mouse F4-4 (2-3months)
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129 SvEvBrd x C57BL/6 heterozygotes
tissue: Soleus muscle
genotype/variation: wild-type
age: Y (2-3 months)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2005-2006, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000
|
| Description |
1-3-82-S-WT
Gene expression data from soleus of wt mouse F4-4 (2-3months)
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and with the Robust Multichip Average (RMA) algorithm in Bioconductor
|
| |
|
| Submission date |
Nov 23, 2011 |
| Last update date |
Jul 05, 2012 |
| Contact name |
Amets Saenz |
| E-mail(s) |
[email protected]
|
| Phone |
+34 943 00 70 61
|
| Fax |
+34 943 00 70 61
|
| Organization name |
Donostia Hospital
|
| Lab |
Unidad Experimental
|
| Street address |
Pº dr Begiristain s/n
|
| City |
San Sebastian |
| State/province |
Gipuzkoa |
| ZIP/Postal code |
20.014 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE33931 |
Expression data from mouse muscle |
|
