|
| Status |
Public on Mar 14, 2012 |
| Title |
K7M2/ezrinT567A-GFP rep1 |
| Sample type |
RNA |
| |
|
| Source name |
K7M2 cell line
|
| Organism |
Mus musculus |
| Characteristics |
cell line: K7M2
overexpression: ezrinT567A-GFP
|
| Growth protocol |
Cells were grown in DMEM medium + 10%FBS at 37 degree with 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA is extracted using Qiagen RNA mini kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix one-cycle eukaryotic target labeling assay protocol
|
| |
|
| Hybridization protocol |
Biotinylated fragmented cRNA was hybridized to GeneChip® Mouse Genome 430 2.0 for 16 hours according to the standard Affymetrix protocol
|
| Scan protocol |
Affymetrix genechip scanner 3000 was used
|
| Data processing |
The .CEL file was exported from Affymetrix AGCC software and the data was processed with RMA using Affymetrix Power Tool
|
| |
|
| Submission date |
Nov 22, 2011 |
| Last update date |
Mar 14, 2012 |
| Contact name |
Chand Khanna |
| E-mail(s) |
[email protected]
|
| Organization name |
NCI
|
| Department |
Pediatric Oncology Branch
|
| Lab |
Tumor and Metastasis Biology Section
|
| Street address |
37 Convent Drive, Suite 2144
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE33897 |
Dysregulation of c-terminal ezrin phosphorylation prevents tumor progression and metastasis and alters cellular metabolism in osteosarcoma |
|
