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Sample GSM8337647 Query DataSets for GSM8337647
Status Public on Dec 15, 2024
Title ChIP_XerA_input_KU216_rep2
Sample type SRA
 
Source name KOD1
Organism Thermococcus kodakarensis
Characteristics cell line: KOD1
strain: Parental strain KU216
chip antibody: None
Extracted molecule genomic DNA
Extraction protocol Crosslinked cells were lysed by sonication, and the extract was clarified by centrifugation. Protein-DNA complexes were purified from the extract with antisera. After reversal of crosslinks, the DNA was extracted with phenol:chloroform:isoamyl alcohol and precipitated in ethanol.
Libraries were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina with Sample Purification Beads.
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina NovaSeq 6000
 
Data processing Data were mapped using Bowtie 2 version 2.3.5.1 (Langmead & Salzberg, 2012). Low-quality alignments (MAPQ < 30) were removed using SAMtools version 1.9 (Li et al., 2009). Generated BAM files were processed using the bamCoverage function (version 3.5.1) of deepTools (Ramirez et al., 2016) to calculate Reads Per Kilobase region per Million mapped reads (RPKM) for genomic bins. Mapped data were processed for peak calling using MACS v3.0.0a6 with the following setting: --fe-cutoff 3, --keep-dup all, --call-summits, -m 1 50.
Assembly: GCA_000009965.1
Supplementary files format and content: bedgraph
Supplementary files format and content: bed
 
Submission date Jun 18, 2024
Last update date Dec 15, 2024
Contact name Naomichi Takemata
Organization name Kyoto University
Department Synthetic Chemistry and Biological Chemistry
Street address Kyoto University, Katsura, Nishikyo-ku
City Kyoto
ZIP/Postal code 615-8510
Country Japan
 
Platform ID GPL28196
Series (1)
GSE267298 Chromosomal domain formation by archaeal SMC, a roadblock protein, and DNA shape [ChIP-seq]
Relations
SRA SRX24972796
BioSample SAMN41900429

Supplementary file Size Download File type/resource
GSM8337647_ChIP_XerA_input_KU216_rep2.bedgraph.gz 284.9 Kb (ftp)(http) BEDGRAPH
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Raw data are available in SRA

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