|
| Status |
Public on Jan 20, 2014 |
| Title |
HFD 663, fat mRNA |
| Sample type |
RNA |
| |
|
| Source name |
fat from female offspring of fathers fed on high-fat diet
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague Dawley
gender: female
tissue: fat
treatment: father fed high-fat diet
|
| Treatment protocol |
Fathers fed on HFD vs. control were mated with females on normal chow. The female offspring's fat transcriptomics were analyzed through microarray analysis.
|
| Growth protocol |
At 15 weeks of age, fathers were mated with normal females consuming chow to generate the F1 offspring.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Rat Gene ST 1.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner GC3000.
|
| Description |
Gene expression of fat from female offspring of fathers fed high-fat chow.
|
| Data processing |
RMA normalization was carried out using Partek Genome Suite v6.4.
|
| |
|
| Submission date |
Nov 08, 2011 |
| Last update date |
Jan 20, 2014 |
| Contact name |
Margaret J Morris |
| E-mail(s) |
[email protected]
|
| Phone |
+61293851560
|
| Organization name |
University of New South Wales
|
| Department |
School of Medical Sciences
|
| Lab |
Head of Pharmacology
|
| Street address |
University of New South Wales
|
| City |
Wallace Wurth Building, Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2052 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6247 |
| Series (2) |
| GSE33551 |
Effects of dietary obesity in fathers on gene expression of fat in the female offspring (mRNA data) |
| GSE33564 |
Effects of dietary obesity in fathers on gene expression of fat in the female offspring |
|
