|
| Status |
Public on Jul 02, 2024 |
| Title |
HN_Water_R2 |
| Sample type |
SRA |
| |
|
| Source name |
Young leaf
|
| Organism |
Malus domestica |
| Characteristics |
tissue: Young leaf
genotype: Gala progeny
treatment: Water
nitrogen diet: HN
nitrogen status: High N
|
| Treatment protocol |
Plants with different N status were obtained after transfer from high N to low N solution for 12 days (N limited), and transferred back on high N (N-resupplied). Reference plants (HN) stayed on high N solution. Plants were ASM treated with 0.4g.L Bion®(50%-ASM, Syngenta) sprayed to run-off after 10 days of N limitation or 24h of N-resupply. Controlled plants were sprayed with demineralized water.
|
| Growth protocol |
Plants were grown under greenhouse conditions (natural photoperiod supplemented with artificial light if needed, 18 °C night and 20–23 °C day), in sub-irrigation devices allowing a fine monitoring of N fertilisation with contrasted nitrate concentrations (high 7 mM N or low 0.5 mM solutions) over the growing period.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using NucleoSpin RNA Plant (Macherey-Nagel GmbH & Co., Düren, Germany) according to the manufacturer's procedure.
Libraries were generated using the Illumina mRNA Stranded protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
RE007
|
| Data processing |
The sequenced reads (ranging from 21.5 to 60 million read pairs per sample) were mapped on the reference transcriptional units from GDDH13 v1.1 using Salmon software (Patro et al., 2017)
Assembly: MJAX00000000.1
Supplementary files format and content: tab-delimited file include raw counts values for each sample
|
| |
|
| Submission date |
Apr 22, 2024 |
| Last update date |
Jul 02, 2024 |
| Contact name |
Mathilde ORSEL |
| E-mail(s) |
[email protected]
|
| Organization name |
IRHS
|
| Street address |
42, rue Georges Morel
|
| City |
Beaucouzé |
| ZIP/Postal code |
49071 |
| Country |
France |
| |
|
| Platform ID |
GPL29987 |
| Series (1) |
| GSE264541 |
Transcriptomic data from Malus domestica young leaves response to Acibenzolar-S-Methyl (ASM) treatment and nitrogen limitation or resupply. |
|
| Relations |
| BioSample |
SAMN41042482 |
| SRA |
SRX24329836 |
