|
| Status |
Public on May 27, 2006 |
| Title |
E13 Sertoli cells XY replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
fetal gonad
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL6/J-Tg92, Age: E13
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from approximately 22,000 EGFP+ sorted XY SSCs using the RNAqueousTM-Micro Kit (Ambion, Inc.) according to the manufacturers instructions.
|
| Label |
biotin
|
| Label protocol |
Approximately 20ng of total RNA was processed to produce biotinylated cDNA targets using the Ovation Biotin (NuGEN) protocol.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures with modifications according to NuGEN protocol
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
sorted E13 Sertoli cells
|
| Data processing |
RMA
|
| |
|
| Submission date |
Nov 03, 2005 |
| Last update date |
May 26, 2006 |
| Contact name |
Gerrit J Bouma |
| E-mail(s) |
[email protected]
|
| Phone |
207-288-6344
|
| Fax |
207-288-6077
|
| Organization name |
The Jackson Laboratory
|
| Street address |
600 Main St
|
| City |
Bar Harbor |
| State/province |
ME |
| ZIP/Postal code |
04609 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE4928 |
Gene expression profile in isolated E13 gonadal somatic support cells and E13 gonads. |
|
