|
| Status |
Public on Feb 15, 2012 |
| Title |
Keratinocytes_ZNF750i_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Primary neonatal keratinocytes
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: primary neonatal keratinocytes
treatment: ZNF750 knock down
|
| Treatment protocol |
siRNA knock down
|
| Growth protocol |
Keratinocytes were grown in Gibco SFM medium till 100% confluent with addition of 1.2 mM Ca2+ for 3 Days to induce differentiation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kits were used to isolate total RNA.
|
| Label |
biotin
|
| Label protocol |
Affymetrix 3' IVT Express Kit
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip Hybridization, Wash, and Stain Kit
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G
|
| Description |
replicate 1
|
| Data processing |
RMA normalization using justRMA package in R 2.11.1
|
| |
|
| Submission date |
Oct 06, 2011 |
| Last update date |
Oct 11, 2022 |
| Contact name |
Douglas Porter |
| Organization name |
Stanford
|
| Department |
Dermatology
|
| Lab |
Khavari
|
| Street address |
269 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE32685 |
ZNF750 Drives Terminal Epidermal Differentiation via Induction of Klf4 |
|
