|
| Status |
Public on Jun 30, 2024 |
| Title |
unassigned_2_WT_02 |
| Sample type |
SRA |
| |
|
| Source name |
RH
|
| Organism |
Toxoplasma gondii RH |
| Characteristics |
cell line: RH
cell type: tachyzoite
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Toxoplasma genomic DNA was extracted using the DNeasy Blood and Tissue kit (Qiagen).
Integrated gRNA sequences were amplified and barcoded by PCR with KOD FX Neo (TOYOBO). Genomic DNA (1μg) was used for the template. The resulting libraries were sequenced on a DNBSEQ-G400RS (MGI).
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
DNBSEQ-G400 |
| |
|
| Description |
Instrument model: DNBSEQ-G400RS
|
| Data processing |
The gRNA sequencing reads were aligned to the gRNA library. The abundance of each gRNA was calculated and normalized to the total number of aligned reads.
Assembly: Unassigend_2 library for CRISPR screen
Supplementary files format and content: text file for each gRNA expresssion
Library strategy: DNA-seq
|
| |
|
| Submission date |
Jan 22, 2024 |
| Last update date |
Jun 30, 2024 |
| Contact name |
Yuta Tachibana |
| E-mail(s) |
[email protected]
|
| Phone |
+81-6-6879-8333
|
| Organization name |
Osaka University
|
| Department |
Research Institute for Microbial Diseases (RIMD)
|
| Lab |
Department of Immunoparasitology
|
| Street address |
3-1, Yamadaoka
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
| |
|
| Platform ID |
GPL34114 |
| Series (1) |
| GSE253885 |
In vivo CRISPR screens for Toxoplasma hyperLOPIT-unassigened proteins_2 |
|
| Relations |
| BioSample |
SAMN39541185 |
| SRA |
SRX23349600 |
