|
| Status |
Public on Sep 10, 2012 |
| Title |
Growth plate cartilage sample 5 |
| Sample type |
RNA |
| |
|
| Source name |
Growth plate cartilage sample 5
|
| Organism |
Homo sapiens |
| Characteristics |
tissue-type: Growth plate cartilage
|
| Treatment protocol |
Healthy explants, all remained untreated
|
| Extracted molecule |
total RNA |
| Extraction protocol |
As detailed in Heinrichs C, et al. (1994) Dexamethasone increases growth hormone receptor messenger ribonucleic acid levels in liver and growth plate. Endocrinology 135: 1113-1118.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Healthy preadolescent cartilage
|
| Data processing |
A customCDF Version 10 entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization. Differential gene expression was analysed based on loglinear mixed model
|
| |
|
| Submission date |
Sep 26, 2011 |
| Last update date |
Sep 11, 2012 |
| Contact name |
Jeroen Leijten |
| Organization name |
University of Twente
|
| Street address |
Drienerlolaan 5
|
| City |
Enschede |
| ZIP/Postal code |
7522NB |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL9828 |
| Series (1) |
| GSE32398 |
Expression data from hyaline cartilages |
|
