|
| Status |
Public on Apr 01, 2012 |
| Title |
66977_ro |
| Sample type |
RNA |
| |
|
| Source name |
tumor
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57BL/6
tissue: tumor
genotype/variation: mutALK
|
| Treatment protocol |
none
|
| Growth protocol |
Mice transgenic for neural crest specific expression of MYCN, mutated ALK or both were monitored until tumor was palpable and sacrificed afterwards.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from tumors or control tissue using the QIAGEN Rneasy mini kit.
|
| Label |
biotin
|
| Label protocol |
Biotin labelling was performed according the manufacturer's recommendations (Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridisation to mouse 430_2 arrays was performed according to the manufacturer's recommendations (Affymetrix).
|
| Scan protocol |
Scanning of chips was performed using a GeneArray scanner (Agilent).
|
| Description |
tumor driven by mutALK
|
| Data processing |
Signal intensities were RMA normalized using PARTEK Genomics Suite, with the .CEL files as input.
|
| |
|
| Submission date |
Sep 26, 2011 |
| Last update date |
Apr 13, 2012 |
| Contact name |
Alexander Schramm |
| E-mail(s) |
[email protected]
|
| Organization name |
University Hospital Essen
|
| Department |
Childrens Hospital
|
| Lab |
Hematology/Oncology Lab
|
| Street address |
Hufelandstr. 55
|
| City |
Essen |
| ZIP/Postal code |
45122 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE32386 |
Expression profiling of murine neuroblastoma in transgenic mice |
|
