|
| Status |
Public on Sep 14, 2011 |
| Title |
RPKM value for R5 |
| Sample type |
SRA |
| |
|
| Source name |
R5 cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: fetal liver cells
|
| Growth protocol |
The RNA is extracted directly from the sorted cells without in vitro culture
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using an RNeasy kit (Qiagen) from freshly sorted cells. The library preparation was performed using polyA enriched RNA according to the manufacturer's instructions (Illumina) and then sequenced on a Solexa sequencing cell.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
RNA-seq expression value for R5 cells
|
| Data processing |
MAQ was used for mapping reads to the mouse mm9 genome. Gene expression values were calculated as Reads Per Kilobase of Exon Model Per Million Mapped Reads (RPKM)
|
| |
|
| Submission date |
Sep 14, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Bill Wong |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
Whitehead Institute
|
| Lab |
BaRC
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL9185 |
| Series (2) |
| GSE32110 |
RNA-seq expression profiles during terminal erythropoiesis |
| GSE32111 |
Gene induction and repression during terminal erythropoiesis are mediated by distinct epigenetic changes. |
|
| Relations |
| BioSample |
SAMN02198145 |
| SRA |
SRX744599 |
