|
| Status |
Public on Dec 06, 2023 |
| Title |
MCD mice-2 |
| Sample type |
RNA |
| |
|
| Source name |
Wild-type mice fed with MCD
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
Sex: male
tissue: Liver
|
| Treatment protocol |
Wild type male C57BL/6J mice at 6 weeks of age were randomly assigned into two groups (normal diet or MCD groups). Mice were fed with normal diet (ND) or MCD for 20 weeks.
|
| Growth protocol |
Mice were maintained at 19-25 ºC with 55 % ± 25 % humidity under a 14-hour light/10-hour dark cycle in specific-pathogen-free are.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from the liver of mice fed with ND or MCD by miRNeasy mini kit (QIAGEN, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA derived from the total RNA and labeled biotin with the GeneChip WT PLUS Reagent Kit (ThermoFisher Scientific, MA, USA).
|
| |
|
| Hybridization protocol |
The single-stranded cDNA was hybridized with a Mouse Gene 2.1 ST Array Strip (ThermoFisher Scientific).
|
| Scan protocol |
The array was scanned by the GeneAtlas system (Affymetrix, MA, USA) to measure the intensities of the microarray spots.
|
| Data processing |
The intensities of the spots were analyzed by the Transcriptome Analysis Console (TAC) software (ThermoFisher Scientific).
|
| |
|
| Submission date |
Dec 06, 2023 |
| Last update date |
Dec 06, 2023 |
| Contact name |
Shuji Sakamoto |
| E-mail(s) |
[email protected]
|
| Organization name |
Kochi University
|
| Department |
Science Research Center
|
| Lab |
Laboratory of Molecular Biology
|
| Street address |
Oko-cho, Kohasu
|
| City |
Nankoku |
| State/province |
Kochi |
| ZIP/Postal code |
783-8505 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17400 |
| Series (2) |
| GSE249485 |
Effect of methionine-chorine-deficient diet (MCD) on whole gene expression in murine liver [mRNA] |
| GSE249487 |
Effect of methionine-chorine-deficient diet (MCD) in murine liver |
|
