|
| Status |
Public on Sep 13, 2011 |
| Title |
Pol2t_2 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
TetO7-POL2
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype/variation: TetO7-POL2
cell cycle phase: S phase
|
| Treatment protocol |
10 μg/ml doxycycline
|
| Growth protocol |
Strains were grown 12-16 hr at 30˚C in YPD. The strains were diluted to O.D 0.05 in YPD +10 μg/ml doxycycline and harvested after 6-7 hr at logaritmic phase .
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA from FACS-sorted cells was extracted using the MasterPure Yeast DNA purification kit
|
| Label |
Cy5
|
| Label protocol |
DNA was digested with DpnII and amplified by four rounds of random-octamer-primed Exo-Klenow DNA synthesis (Invitrogen BioPrime ArrayCGH amplification kit) with amino-allyl modified nucleotides. DNA was labeled with Cy dyes
|
| |
|
| Channel 2 |
| Source name |
TetO7-POL2
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype/variation: TetO7-POL2
cell cycle phase: G2 phase
|
| Treatment protocol |
10 μg/ml doxycycline
|
| Growth protocol |
Strains were grown 12-16 hr at 30˚C in YPD. The strains were diluted to O.D 0.05 in YPD +10 μg/ml doxycycline and harvested after 6-7 hr at logaritmic phase .
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA from FACS-sorted cells was extracted using the MasterPure Yeast DNA purification kit
|
| Label |
Cy3
|
| Label protocol |
DNA was digested with DpnII and amplified by four rounds of random-octamer-primed Exo-Klenow DNA synthesis (Invitrogen BioPrime ArrayCGH amplification kit) with amino-allyl modified nucleotides. DNA was labeled with Cy dyes
|
| |
|
| |
| Hybridization protocol |
Agilent yeast ChIP-on-chip180Kx4 arrays
|
| Scan protocol |
Agilent microarray scanner
Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
|
| Data processing |
Matlab was used to read the files, background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Sep 12, 2011 |
| Last update date |
Sep 13, 2011 |
| Contact name |
Ariel Gispan |
| E-mail(s) |
[email protected]
|
| Organization name |
Weizmann Institute of Science
|
| Department |
Molecular Genetics
|
| Lab |
Barkai
|
| Street address |
P.O. Box 26
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL14566 |
| Series (1) |
| GSE32002 |
Replication Timing in Yeast: CGH microarrays: S vs G1 cells in various stress conditions |
|
