|
Status |
Public on Sep 09, 2011 |
Title |
Rad53_2 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
rad53Δ, sml1 Δ
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
genotype/variation: rad53Δ, sml1 Δ cell cycle phase: S phase
|
Growth protocol |
Strains were grown 12-16 hr at 30˚C in YPD. The strains were diluted to O.D 0.1 in YPD and harvested after 5 hr at logaritmic phase .
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA from FACS-sorted cells was extracted using the MasterPure Yeast DNA purification kit
|
Label |
Cy5
|
Label protocol |
DNA was digested with DpnII and amplified by four rounds of random-octamer-primed Exo-Klenow DNA synthesis (Invitrogen BioPrime ArrayCGH amplification kit) with amino-allyl modified nucleotides. DNA was labeled with Cy dyes
|
|
|
Channel 2 |
Source name |
rad53Δ, sml1 Δ
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
genotype/variation: rad53Δ, sml1 Δ cell cycle phase: G1 phase
|
Growth protocol |
Strains were grown 12-16 hr at 30˚C in YPD. The strains were diluted to O.D 0.1 in YPD and harvested after 5 hr at logaritmic phase .
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA from FACS-sorted cells was extracted using the MasterPure Yeast DNA purification kit
|
Label |
Cy3
|
Label protocol |
DNA was digested with DpnII and amplified by four rounds of random-octamer-primed Exo-Klenow DNA synthesis (Invitrogen BioPrime ArrayCGH amplification kit) with amino-allyl modified nucleotides. DNA was labeled with Cy dyes
|
|
|
|
Hybridization protocol |
Agilent yeast ChIP-on-chip44Kx4 arrays
|
Scan protocol |
Agilent microarray scanner Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
|
Data processing |
Matlab was used to read the files, background subtraction and LOWESS normalization.
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|
|
Submission date |
Sep 08, 2011 |
Last update date |
Sep 09, 2011 |
Contact name |
Ariel Gispan |
E-mail(s) |
[email protected]
|
Organization name |
Weizmann Institute of Science
|
Department |
Molecular Genetics
|
Lab |
Barkai
|
Street address |
P.O. Box 26
|
City |
Rehovot |
ZIP/Postal code |
76100 |
Country |
Israel |
|
|
Platform ID |
GPL10930 |
Series (1) |
GSE32002 |
Replication Timing in Yeast: CGH microarrays: S vs G1 cells in various stress conditions |
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