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Status
Public on Mar 29, 2006
Title
Expression data of rectal cancer T80
Sample type
RNA
Source name
Patient ID T80
Organism
Homo sapiens Characteristics
Tissue: rectal cancer
Extracted molecule
total RNA
Extraction protocol
Surgically isolated human colorectal cancer tissues were immediately frozen in liquid nitrogen. Tissues were lysed and total RNA was extracted using the Sepasol-RNA I (WAKO, Osaka, Japan) according to the manufacturer's instructions. Total RNA was then purified using an RNeasy column (Qiagen, Austin, TX).
Label
biotin
Label protocol
Approximately 5 ug of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
test-responder
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Oct 21, 2005
Last update date
Mar 16, 2009
Contact name
Toshiaki Watanabe
Phone
81-3-5800-8653
Organization name
University of Tokyo
Department
Department of Surgical Oncology
Street address
7-3-1, Hongo
City
Bunkyo-ku
State/province
Tokyo
ZIP/Postal code
113-8655
Country
Japan
Platform ID
GPL8300 Series (1)
GSE3493 Rectal Cancer Cells and Radiation-sensitivity
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
Data table ID_REF VALUE ABS_CALL 100_g_at
444.9
A
1000_at
1849.2
P
1001_at
292.7
M
1002_f_at
47.2
A
1003_s_at
263.8
A
1004_at
405.4
A
1005_at
892.8
P
1006_at
7.1
A
1007_s_at
2011.6
P
1008_f_at
9901.7
P
1009_at
1464.9
P
101_at
54.1
A
1010_at
26.3
A
1011_s_at
301
P
1012_at
8.6
A
1013_at
59.3
A
1014_at
576.1
A
1015_s_at
89.2
A
1016_s_at
131.6
A
1017_at
113.2
A
12625 205 Kbytes .Supplementary data files not provided
