GEO help: Mouse over screen elements for information.
Status
Public on Mar 29, 2006
Title
Expression data of rectal cancer TKR25T
Sample type
RNA
Source name
Patient ID TKR25T
Organism
Homo sapiens Characteristics
Tissue: rectal cancer
Extracted molecule
total RNA
Extraction protocol
Surgically isolated human colorectal cancer tissues were immediately frozen in liquid nitrogen. Tissues were lysed and total RNA was extracted using the Sepasol-RNA I (WAKO, Osaka, Japan) according to the manufacturer's instructions. Total RNA was then purified using an RNeasy column (Qiagen, Austin, TX).
Label
biotin
Label protocol
Approximately 5 ug of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
training-non-responder
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Oct 21, 2005
Last update date
Mar 16, 2009
Contact name
Toshiaki Watanabe
Phone
81-3-5800-8653
Organization name
University of Tokyo
Department
Department of Surgical Oncology
Street address
7-3-1, Hongo
City
Bunkyo-ku
State/province
Tokyo
ZIP/Postal code
113-8655
Country
Japan
Platform ID
GPL8300 Series (1)
GSE3493 Rectal Cancer Cells and Radiation-sensitivity
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
Data table ID_REF VALUE ABS_CALL 100_g_at
455.4
A
1000_at
988
P
1001_at
39.6
A
1002_f_at
26.5
A
1003_s_at
177
A
1004_at
201.9
A
1005_at
304.8
M
1006_at
36.8
A
1007_s_at
1743.9
P
1008_f_at
5361.5
P
1009_at
3240.1
P
101_at
94.2
A
1010_at
11.3
A
1011_s_at
626.5
P
1012_at
27.3
A
1013_at
69.1
P
1014_at
416.9
M
1015_s_at
75.2
A
1016_s_at
74.4
A
1017_at
65.3
A
12625 204 Kbytes .Supplementary data files not provided
