GEO help: Mouse over screen elements for information.
Status
Public on Mar 29, 2006
Title
Expression data of rectal cancer TKR23T
Sample type
RNA
Source name
Patient ID TKR23T
Organism
Homo sapiens Characteristics
Tissue: rectal cancer
Extracted molecule
total RNA
Extraction protocol
Surgically isolated human colorectal cancer tissues were immediately frozen in liquid nitrogen. Tissues were lysed and total RNA was extracted using the Sepasol-RNA I (WAKO, Osaka, Japan) according to the manufacturer's instructions. Total RNA was then purified using an RNeasy column (Qiagen, Austin, TX).
Label
biotin
Label protocol
Approximately 5 ug of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
training-non-responder
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Oct 21, 2005
Last update date
Mar 16, 2009
Contact name
Toshiaki Watanabe
Phone
81-3-5800-8653
Organization name
University of Tokyo
Department
Department of Surgical Oncology
Street address
7-3-1, Hongo
City
Bunkyo-ku
State/province
Tokyo
ZIP/Postal code
113-8655
Country
Japan
Platform ID
GPL8300 Series (1)
GSE3493 Rectal Cancer Cells and Radiation-sensitivity
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
Data table ID_REF VALUE ABS_CALL 100_g_at
315.3
A
1000_at
1042.2
P
1001_at
84.4
A
1002_f_at
15.8
A
1003_s_at
162.8
A
1004_at
282.9
A
1005_at
477.7
M
1006_at
9
A
1007_s_at
1859.4
P
1008_f_at
5824.4
P
1009_at
3655.9
P
101_at
23.5
A
1010_at
16.4
A
1011_s_at
628.2
P
1012_at
31.4
A
1013_at
28.6
A
1014_at
519.6
A
1015_s_at
35.1
A
1016_s_at
73.6
A
1017_at
47.1
A
12625 205 Kbytes .Supplementary data files not provided
