|
| Status |
Public on Oct 25, 2005 |
| Title |
Radium Human 22k v2.0, NOWAC35 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Stratagen reference
|
| Organism |
Homo sapiens |
| Characteristics |
Stratagen reference
|
| Extracted molecule |
total RNA |
| Label |
Cy3
|
| Label protocol |
Agilent Low RNA Input Fluorescent Linear Amplification Kit (Product Number 5184-3523).300 ng of total RNA were amplified and labeled
|
| |
|
| Channel 2 |
| Source name |
NOWAC35
|
| Organism |
Homo sapiens |
| Characteristics |
no HRT use 16.9 pmol/ug cRNA
|
| Extracted molecule |
total RNA |
| Label |
Cy5
|
| Label protocol |
Agilent Low RNA Input Fluorescent Linear Amplification Kit (Product Number 5184-3523).300 ng of total RNA were amplified and labeled
|
| |
|
| |
| Hybridization protocol |
use of stabilization and drying solution, Agilent, Palo Alto, CA
|
| Description |
Whole blood samples collectedin PAXgene tubes
|
| Data processing |
Agilent Feature Extraction software version A.7.5.1 (default settings)
|
| |
|
| Submission date |
Oct 20, 2005 |
| Last update date |
Oct 21, 2005 |
| Contact name |
Vanessa Dumeaux |
| E-mail(s) |
[email protected]
|
| Organization name |
"The Norwegian Women and Cancer project"
|
| Street address |
University of Tromsoe
|
| City |
Tromsoe |
| ZIP/Postal code |
9037 |
| Country |
Norway |
| |
|
| Platform ID |
GPL887 |
| Series (1) |
| GSE3492 |
Whole-blood samples from women exposed to hormone replacement therapy |
|
| Data table header descriptions |
| ID_REF |
|
| VALUE |
Log (base 10) of the ratio of the processed signals of Channel 2 to Channel 1 |
| Cy5MeanSignal |
Raw mean signal of feature in Cy5 channel |
| Cy3MeanSignal |
Raw mean signal of feature in Cy3 channel |
| Cy5BGMedianSignal |
Median local background signal (local to corresponding feature) for Cy5 channel |
| Cy3BGMedianSignal |
Median local background signal (local to corresponding feature) for Cy3 channel |
| Cy5BGMeanSignal |
Mean local background signal (local to corresponding feature) for Cy5 channel |
| Cy3BGMeanSignal |
Mean local background signal (local to corresponding feature) for Cy3 channel |
| Cy5ProcessedSignal |
Dye-normalized signal for Cy5 channel after surrogate algorithm. Used for computation of VALUE |
| Cy3ProcessedSignal |
Dye-normalized signal for Cy3 channel after surrogate algorithm. Used for computation of VALUE |
