|
| Status |
Public on Nov 21, 2024 |
| Title |
Tape_stripped_skin_replicate_6 |
| Sample type |
SRA |
| |
|
| Source name |
skin
|
| Organism |
Mus musculus |
| Characteristics |
tissue: skin
genotype: WT
strain: Balb/c
group: Tape stripped
|
| Treatment protocol |
Skin samples were stored in RNAlater and store at -80 C until RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed using RNeasy Mini Kit (Qiagen) and RNA was retrotranscribed using SuperScript VILO cDNA Synthesis Kit (ThermoFisher Scientific)
cDNA libraries were prepare using Ion AmpliSeqTM Transcriptome Mouse Gene Expression Panel, Chef-Ready Kit in the the Ion Chef System
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Ion Torrent S5 |
| |
|
| Data processing |
Run metrics, read alignment and detection of on-target transcripts was done using Torrent Suite Software 5.12.1 according to standard analysis procedures
CHP files were generated using Ion AmpliSeq RNA plug-in
Assembly: mm9
Supplementary files format and content: CHP format. Processed data files (.chp) contain normalized counts (read per million) .
|
| |
|
| Submission date |
Nov 06, 2023 |
| Last update date |
Nov 21, 2024 |
| Contact name |
Manuel Leyva-Castillo |
| E-mail(s) |
[email protected]
|
| Phone |
617-919-2465
|
| Organization name |
Boston Children's Hospital
|
| Street address |
300 Longwood Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL25723 |
| Series (2) |
| GSE247065 |
Effect of Itape stripping in skin gene expression [TS_skin] |
| GSE247066 |
Skin gene expression |
|
| Relations |
| BioSample |
SAMN38123810 |
| SRA |
SRX22386324 |
