|
| Status |
Public on Feb 19, 2013 |
| Title |
Skeletal Muscle 20mgkg-1 rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Skeletal Muscle
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Skeletal Muscle
mouse number: 19
dosage: 20 mg/kg
treatment: PPM-201
ast (u/l): 1300.4
creatinine (umol/l): 6
bun (mmol/l): 7.64
ldh (u/l): 3172
ck (u/l): 2544
rna integrity number (rin): 9.5
strain: C57BL/6J
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed in random batches by polytron homogenisation of tissue in Trizol (cat. 15596-026, Invitrogen) followed by phenol chloroform extraction. RNAs were quantitated and qualified using Nanodrop ND8000 (Thermo Scientific) and 2100 Bioanalyser (Agilent). Prior to cDNA probe generation RNAs were subjected to sample clean up and DNAse I treatment using RNEasy Micro (Qiagen) before repeat quantitation and qualification.
|
| Label |
biotin
|
| Label protocol |
50ng RNA from each sample were used to generate complementary DNA strands using Ovation RNA Amplification System V2 (cat. 3100-12, Nugen) and 3.75ug of each cDNA were fragmented and biotin labelled using Encore™ Biotin Module (cat. 4200-12, Nugen).
|
| |
|
| Hybridization protocol |
Probe quality and fragmentation were confirmed using Bioanalyser 2100 prior to microarray hybridisation. Fragmented and labelled probes were hybridised for 18 hours onto Affymetrix GeneChip® Mouse Genome 430 2.0 Array (cat. 900496, Affymetrix) using GeneChip Hybridization, Wash, and Stain Kit (cat. 900720-900722, Affymetrix).
|
| Scan protocol |
Microarrays were scanned using Genechip Scanner 3000 7G.
|
| Description |
2008-03-14
|
| Data processing |
The data in the matrix table were generated from raw CEL files using RMA normalisation method in Partek GS 6.5 software.
|
| |
|
| Submission date |
Aug 22, 2011 |
| Last update date |
Feb 19, 2013 |
| Contact name |
Manikhandan A V Mudaliar |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Glasgow
|
| Department |
College of Medical, Veterinary and Life Sciences
|
| Lab |
Institute of Infection, Immunity and Inflammation
|
| Street address |
Room B528, Sir Graeme Davies Building, 120 University Place
|
| City |
Glasgow |
| State/province |
Scotland |
| ZIP/Postal code |
G12 8TA |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE31561 |
Transcriptional analysis of organ-specific toxicity induced by a panPPAR agonist in mice: Identification of organ-specific toxicity biomarkers |
|
