|
| Status |
Public on Apr 01, 2014 |
| Title |
Mut P4 1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse uteri (SRC-2 mutant), progesterone (P4)-treated
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: SRC-2 mutant
tissue: uteri
|
| Treatment protocol |
Mice were ovariectomized at 6 weeks and after 2 weeks mice were either treated with sesame oil (vehicle) or 1 mg of P4.
|
| Growth protocol |
Microarray analysis was performed using eighteen SRC-2flox/flox (SRC-2f/f) and eighteen PRCre/+ SRC-2flox/flox (SRC-2d/d) mice. Mice were maintained in the designated animal care facility at the Baylor College of Medicine and mice were handled in accordance with Institutional Guideline for the Care and handling of laboratory animals.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After six hours uteri from each mouse were collected and total RNA was isolated using QIAGEN RNeasy total RNA isolation kit. RNA from three mice per genotype per treatment were pooled and assigned as one sample (three samples per genotype per treatment). Quality of all RNA samples were analyzed with a Bioanalyzer 2100 (AgilentTechnologies, Palo Alto, CA) before microarray hybridization.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized on Mouse430_2 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
|
| Description |
mouse uteri (SRC-2 wild-type), vehicle-treated, pooled rep 1
|
| Data processing |
The data were analyzed with dChip version2006 using Affymetrix default analysis settings, PM-MM model, and quantile normalization method.
|
| |
|
| Submission date |
Aug 16, 2011 |
| Last update date |
Apr 01, 2014 |
| Contact name |
Chad Creighton |
| E-mail(s) |
[email protected]
|
| Organization name |
Baylor College of Medicine
|
| Department |
Biostatistics, Ducan Cancer Center
|
| Street address |
One Baylor Plaza, Mail Stop: BCM305
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE31406 |
Gene expression analyses of PR action in the uteri of SRC-2 mutant mice |
|
