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Sample GSM773327 Query DataSets for GSM773327
Status Public on Mar 09, 2012
Title IL28B-24_2
Sample type RNA
 
Source name IL28B-24_2
Organism Homo sapiens
Characteristics tissue: liver
agent: IL28B
time: 24
Treatment protocol RNA was isolated after 6, 12, and 24 h treatment with IFN-α (10 U/mL) or IL28B (25 ng/mL)
Growth protocol Primary Human Hepatocytes Were Isolated and Plated on 12 well plates at the University of Pittsburgh in the Laboratory of Dr. Stehphen Strom and provided through the NIH funded Liver Tissue and Cell Distribution System (LTCDS)
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Data processing RMA nomanization by software Partek
 
Submission date Aug 03, 2011
Last update date Mar 09, 2012
Contact name WeiPing Chen
E-mail(s) weipingChen@niddk.nih.gov
Phone 301-496-0175
Organization name NIDDK/NIH
Department GCL
Lab Genomics Core Lab
Street address Bldg 8, Room 1A11, NIDDK/NIH
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL570
Series (1)
GSE31193 A Robust Induction of Type III Interferons and Chemokines Defines a Unique Pattern of Hepatic Innate Immunity in Response to Hepatitis C Virus Infection

Data table header descriptions
ID_REF
VALUE rma normalized

Data table
ID_REF VALUE
1007_s_at 8.35471
1053_at 9.20293
117_at 5.65951
121_at 7.90598
1255_g_at 2.89188
1294_at 10.0202
1316_at 7.81052
1320_at 5.87977
1405_i_at 3.33204
1431_at 9.898
1438_at 4.69578
1487_at 10.1526
1494_f_at 11.3957
1552256_a_at 10.1138
1552257_a_at 10.8767
1552258_at 4.42739
1552261_at 4.85948
1552263_at 7.71564
1552264_a_at 8.56146
1552266_at 3.31824

Total number of rows: 54675

Table truncated, full table size 998 Kbytes.




Supplementary file Size Download File type/resource
GSM773327.CEL.gz 7.9 Mb (ftp)(http) CEL
GSM773327.CHP.gz 7.0 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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