|
| Status |
Public on May 25, 2012 |
| Title |
rlm1 mutant exposed to Congo Red 4 hours_2 |
| Sample type |
RNA |
| |
|
| Source name |
rlm1 Congo Red 4h
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: BY4741
genotype/variation: rlm1 mutant
|
| Treatment protocol |
Saccharomyces cerevisiae strain BY4741 was grown overnight and dilluted to 0.2 OD, allowed to grow until 0.5 and then was grown with Congo Red for 4 hours
|
| Growth protocol |
Saccharomyces cerevisiae strain BY4741 was grown overnight and dilluted to 0.2 OD, allowed to grow until 0.5 and then was grown for 4 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from exponentially growing cells by the “mechanical disruption protocol” using the RNeasy kit (Qiagen, Hilden, Germany), following the instructions of the manufacturer. RNA concentrations were determined by measuring absorbance at 260 nm. RNA purity and integrity were assessed using RNA Nano Labchips in an Agilent 2100B Bioanalyzer (Agilent Technologies, Palo Alto, CA) following the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Following affymetrix labelling instructions
|
| |
|
| Hybridization protocol |
Following affymetrix hybridation instructions
|
| Scan protocol |
Following affymetrix scanning instructions
|
| Description |
All the procedure for microarrays experiments was carried out following the instructions of affymetrix for each of the steps
|
| Data processing |
CEL files from scaner were analyzed using GCRMA algorithm from Bioconductor software. Only yeast probes were included in the final analysis.
|
| |
|
| Submission date |
Aug 03, 2011 |
| Last update date |
May 25, 2012 |
| Contact name |
Javier Arroyo |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
0034-913941746
|
| URL |
http://ucm.es/info/mfar
|
| Organization name |
Facultad de Farmacia (UCM)
|
| Department |
Microbiologia II
|
| Lab |
U4-Javier Arroyo
|
| Street address |
Plaza Ramon y Cajal S/N
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE31176 |
Expression data from yeast (wild type, rlm1 and swi3 mutants) exposed to Congo Red |
|
