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Sample GSM7713108 Query DataSets for GSM7713108
Status Public on Aug 17, 2023
Title Zfp36aa/aa derived Macrophage replicate 1 LPS and dexamethasone Stimulated 4 hour
Sample type RNA
 
Source name Bone Marrow M-CSF dervied Macrophage
Organism Mus musculus
Characteristics strain: C57BL/6
genotype/variation: Zfp36 mutant (aa/aa) macrophage
treatment: Cells were stimulated with 10ng/mL LPS and 100nM dexamethasone for 4 hours prior to RNA isolation
replicate: 1
Treatment protocol Cells were either left untreated or stimulated with 10ng/ml LPS for 1-4 hours, or 10ng/ml LPS plus 100nM dexamethasone for 4 hours, prior to RNA isolation.
Growth protocol Bone Marrow differentiated using 100ng/mL M-CSF for 5 days
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using QIAGEN RNA isolation kit and ZYMO RNA concentrator kit following manufacturer's instructions
Label Cy3
Label protocol Total RNA was converted to labelled cRNA with Cy3 or Cy5 respectively using the Low Input Quick Amp Labeling Kit, Two-Color (Agilent Technologies).
 
Hybridization protocol hybridized onto SurePrint G3 Mouse GE 8x60K slides
Scan protocol Scanned using a G2505C scanner (Agilent Technologies).
Description hybridized onto SurePrint G3 Mouse GE 8x60K slides
Data processing Agilent Feature Extraction Software (10.7.3.1) using default parameters using the Cy3 colour only
 
Submission date Aug 16, 2023
Last update date Aug 17, 2023
Contact name John Daniel O'Neil
E-mail(s) [email protected]
Organization name University of Birmingham
Department Immunity and Infection
Lab Rheumatology Research Group
Street address Queen Elizabeth Hospital
City Birmingham
ZIP/Postal code B15 2WB
Country United Kingdom
 
Platform ID GPL10787
Series (1)
GSE68449 Dual Specificity Phosphatase 1 and Tristetraprolin cooperate to regulate macrophage responses to LPS and dexamethasone

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
A_30_P01017425 5.55783
A_30_P01017426 6.50853
A_30_P01017427 3.00262
A_30_P01017428 5.6179
A_30_P01017429 2.82053
A_30_P01017430 2.94371
A_30_P01017431 2.85775
A_30_P01017432 6.00749
A_30_P01017433 5.38954
A_30_P01017434 5.88353
A_30_P01017435 2.80148
A_30_P01017436 2.73513
A_30_P01017437 8.5763
A_30_P01017438 2.78233
A_30_P01017439 7.65563
A_30_P01017440 2.75191
A_30_P01017441 3.0112
A_30_P01017442 7.27323
A_30_P01017443 10.543
A_30_P01017444 3.79003

Total number of rows: 55681

Table truncated, full table size 1191 Kbytes.




Supplementary file Size Download File type/resource
GSM7713108_AA1-4LPS+dex.txt.gz 20.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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