|
| Status |
Public on Aug 17, 2023 |
| Title |
Wild Type derived Macrophage replicate 2 LPS and dexamethasone Stimulated 4 hour |
| Sample type |
RNA |
| |
|
| Source name |
Bone Marrow M-CSF dervied Macrophage
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: wild type derived macrophage
treatment: Cells were stimulated with 10ng/mL LPS and 100nM dexamethasone for 4 hours prior to RNA isolation
replicate: 2
|
| Treatment protocol |
Cells were either left untreated or stimulated with 10ng/ml LPS for 1-4 hours, or 10ng/ml LPS plus 100nM dexamethasone for 4 hours, prior to RNA isolation.
|
| Growth protocol |
Bone Marrow differentiated using 100ng/mL M-CSF for 5 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using QIAGEN RNA isolation kit and ZYMO RNA concentrator kit following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Total RNA was converted to labelled cRNA with Cy3 or Cy5 respectively using the Low Input Quick Amp Labeling Kit, Two-Color (Agilent Technologies).
|
| |
|
| Hybridization protocol |
hybridized onto SurePrint G3 Mouse GE 8x60K slides
|
| Scan protocol |
Scanned using a G2505C scanner (Agilent Technologies).
|
| Description |
hybridized onto SurePrint G3 Mouse GE 8x60K slides
|
| Data processing |
Agilent Feature Extraction Software (10.7.3.1) using default parameters using the Cy3 colour only
|
| |
|
| Submission date |
Aug 16, 2023 |
| Last update date |
Aug 17, 2023 |
| Contact name |
John Daniel O'Neil |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Birmingham
|
| Department |
Immunity and Infection
|
| Lab |
Rheumatology Research Group
|
| Street address |
Queen Elizabeth Hospital
|
| City |
Birmingham |
| ZIP/Postal code |
B15 2WB |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10787 |
| Series (1) |
| GSE68449 |
Dual Specificity Phosphatase 1 and Tristetraprolin cooperate to regulate macrophage responses to LPS and dexamethasone |
|
