|
| Status |
Public on Dec 06, 2005 |
| Title |
Human Hair Follicle Sub-Bulge#1 |
| Sample type |
RNA |
| |
|
| Source name |
Human Scalp
|
| Organism |
Homo sapiens |
| Characteristics |
Transverse frozen sections of human scalp were made. After precisely defining the different anatomical locations of individual hair follicles, laser capture microdissection was used to isolate the desired hair follicle cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Picopure RNA isolation kit (Arcturas) and DNAse digestion (Qiagen)
Quality assessed by Agilent 2100 bioanalyzer and a RNA 6000 Pico LabChip kit (Agilent)
|
| Label |
Biotin
|
| Label protocol |
T7-primer based two round linear RNA amplification protocol: "Genechip Eukaryotic Small Sample Target Labeling Assay Version II" (equivalent to the GeneChip Two-Cycle cDNA Synthesis kit, Affymetrix)
|
| |
|
| Description |
Affymetrix protocol
|
| Data processing |
MASv5.0
|
| |
|
| Submission date |
Oct 06, 2005 |
| Last update date |
Oct 06, 2005 |
| Contact name |
Jonathan Vogel |
| E-mail(s) |
[email protected]
|
| Phone |
301 496-9002
|
| Organization name |
National Cancer Institute; NIH
|
| Department |
Dermatology
|
| Street address |
Bldg. 10 / Room 12N260
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892-1908 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE3419 |
Characterization and isolation of stem cell enriched human hair follicle bulge cells |
|
